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For rabies tracing, a total of 500 nl, containing a 1:1 mixture of AAV5-FLEx loxP -TC (UNC Vector Core, titre ≥ 2.4×10 12 gc ml −1 ) and AAV8-FLEx loxP -RABV-G (UNC Vector Core, titre ≥ 1.0 × 10 12 gc ml −1 ), was injected into the VTA of DAT-Cre mice
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Regarding synthesis of this unimolecular dual-agonist, as Gcg amino acid positions 3, 10, and 12 are of critical importance to GcgR activation, and as the N-terminal sequences of both Gcg and GLP-1 have capacity to bind the GLP-1R, the first 15 amino acids of the Gcg base sequence were left unmodified
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