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Measurement of calcium response Ca 2+ imaging was performed under continuous perfusion of NG neurons with Krebs–Ringer-Henseleit buffer (Composition in mmol L −1 : NaCl 119, KCl 4.74, CaCl 2 2.54, MgCl 2 1.19, KH 2 PO 4 1.19, NaHCO 3 25, HEPES 10, pH 7.4) at a flow rate of 600 μl/min at 37 °C
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This stored glycogen can be converted back into glucose and released into the bloodstream when the body needs it
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