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The primer sequences were the following: sense: 5′-ACCGCCCTGAGATTACTTTTCTG-3′, antisense: 5′-AGTTCTCTTTCCAGGTTCACCAC-3′ and probe: 5′-6-FAM™-CGCAGTCACGCAACCTGGATTACAA-ZEN-3′ (Iowa Black® FQ)

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