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The antibody Fab7F38 appears to function by providing enhanced soluble surface area for crystal lattice packing, and indeed Fab7F38-bound GLP-1R can also assume an active conformation as shown by EM studies (see below), suggesting that Fab7F38 does not interfere with the conformational flexibility of the ECD during crystallization
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Tirzepatide was a 39-amino acid linear synthetic peptide that shared19 amino acids with human GIP, conjugated to a C20 fatty diacid moiety via a linker connected to the lysine residue at position 20
